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LP3IIG2細(xì)胞

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產(chǎn)品名稱: LP3IIG2細(xì)胞
產(chǎn)品型號(hào): LP3IIG2
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔

簡(jiǎn)單介紹

LP3IIG2細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o(wú)菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無(wú)菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來(lái)源和培養(yǎng)基配制是減低污染之*好方法。LP3IIG2細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


LP3IIG2細(xì)胞  的詳細(xì)介紹

LP3IIG2細(xì)胞

ATCC Number: HB-8472?

是否是腫瘤細(xì)胞: 0

物種來(lái)源: 小鼠

數(shù)量: 大量

生長(zhǎng)狀態(tài): 懸浮生長(zhǎng)

運(yùn)輸方式: 凍存運(yùn)輸

細(xì)胞形態(tài): **樣

**類型: IgG2a

LP3IIG2細(xì)胞Designations: LP3IIG2

Depositors: Genetic Systems Corp.

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: LP3IIG2細(xì)胞immunoglobulin; monoclonal antibody; against Legionella pneumophila

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with Legionella pneumophila.

Spleen cells were fused with NS-1 myeloma cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.

LP3IIG2細(xì)胞Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 3893: Goldstein LC, Gosting LH. LP3IIG2細(xì)胞Method and composition for the detection and diagnosis of Legionella pneumophila. US Patent 4,851,333 dated Jul 25 1989

滬公網(wǎng)安備 31011702004356號(hào)

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