THLE-2細(xì)胞

年限： 1 year infant

運輸方式： 凍存運輸

ATCC Number： TIB-202?

相關(guān)**： 其他**

器官來源： 外周血

細(xì)胞形態(tài)： 單核細(xì)胞/巨噬細(xì)胞

細(xì)胞類型： 其他細(xì)胞類型

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 人

THLE-2細(xì)胞生長狀態(tài)： 懸浮生長

Designations: THP-1

Depositors: S Tsuchiya

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: monocyte

Source: Organ: peripheral blood

Disease: acute monocytic leukemia

Cell Type: monocyte;

Cellular Products: lysozyme

Permits/Forms:THLE-2細(xì)胞 In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Receptors: complement (C3), expressed [58053]

Fc, expressed

Antigen Expression: HLA A2, A9, B5, DRw1, DRw2 [58053]

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,13

D13S317: 13

D16S539: 11,12

D5S818: 11,12

D7S820: 10

THO1: 8,9.3

TPOX: 8,11

vWA: 16

Age: 1 year infant

Gender: male

THLE-2細(xì)胞Comments: The cells are phagocytic (for both latex beads and sensitized erythrocytes) and lack surface and cytoplasmic immunoglobulin.

Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-4 X 10(5) viable cells/ml. Subcultutre when cell concentration reaches 8X105) cells/ml.Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: approximately 26 hrs

Related Products: THLE-2細(xì)胞purified RNA:ATCC TIB-202R

purified DNA:ATCC TIB-202D

Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001

Recommended serum: ATCC 30-2020

Cell culture tested DMSO: ATCC 4-X

References: 22193: Tsuchiya S, et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 42: 1530-1536, 1982. PubMed: 6949641

22285: Skubitz KM, et al. Human granulocyte surface molecules identified by murine monoclonal antibodies. J. Immunol. 131: 1882-1888, 1983. PubMed: 6619543

32286: Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019

32351: Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475

32395: Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679

32466: Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116

33031: Hsu HY, et al. Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-alpha is transcriptionally and post-transcriptionally regulated. J. Biol. Chem. 271: 7767-7773, 1996. PubMed: 8631819

33088: Lucas M, Mazzone T. Cell surface proteoglycans modulate net synthesis and secretion of macrophage apolipoprotein E. J. Biol. Chem. 271: 13454-13460, 1996. PubMed: 8662812

33134: Sando GN, et al. Induction of ceramide glucosyltransferase activity in cultured human keratinocytes. J. Biol. Chem. 271: 22044-22051, 1996. PubMed: 8703011

33141: Ollivier V, et al. Elevated cyclic AMP inhibits NF-kappaB-mediated transcription in human monocytic cells and endothelial cells. J. Biol. Chem. 271: 20828-20835, 1996. THLE-2細(xì)胞PubMed: 8702838

58053: Tsuchiya S, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int. J. Cancer 26: 171-176, 1980. PubMed: 6970727