132-1C8細(xì)胞

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

**類型： IgG1 kappa

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

年限： *****

品系： 129GIX+

ATCC Number： CRL-2737?

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 脾

132-1C8細(xì)胞Designations: 132-1C8

Depositors: National Cancer Institute

Isotype: IgG1 kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Organ: spleen

Strain: 129GIX+

Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against a synthetic MYB oncogene peptide

Permits/Forms: 132-1C8細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: *****

Gender: male

Comments: Animals were immunized with a synthetic polypeptide with the sequence RHYDEDPEKEKRIKELEL corresponding to peptides 282 to 300 of the MYB oncogene. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with the synthetic MYB oncogene peptide. In immunoblot assays, the antibody recognizes proteins of approximately 80 kd and 60 kd in extracts from MSTF cells (mink lung cells transformed by fes). In human tumor (MOLT 4), a 60 kd protein is detected as well as two proteins of 40 and 45 kd. These two proteins of 40 and 45 kd are also detected in human brain extracts. In BM2 cells, proteins of 50 kd and 20 kd are detected.

132-1C8細(xì)胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.

Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: 132-1C8細(xì)胞Complete growth medium supplemented with 5% (v/v) DMSOComplete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase