LC-540細胞

數(shù)量： 大量

ATCC Number： CCL-43?

相關(guān)**： 其他**

是否是腫瘤細胞： 0

物種來源： 大鼠

運輸方式： 凍存運輸

生長狀態(tài)： 貼壁生長

細胞形態(tài)： 上皮樣

品系： Fischer

年限： *****

LC-540細胞器官來源： 睪丸

Designations: LC-540

Depositors: A Steinberger

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Organ: testis

Strain: Fischer

Disease: Leydig cell tumor

Permits/Forms: LC-540細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: October, 1969

Virus Susceptibility: Herpes simplex virus

Vaccinia virus

Vesicular stomatitis virus

Human poliovirus 1

Virus Resistance: poliovirus 1

Tumorigenic: Yes

Cytogenetic Analysis: modal number = 42; range = 40 to 43.

Approximately 21% of the cells examined were aneuploids. No other structural abnormalities were noted.

Age: *****

Gender: male

Propagation: LC-540細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: LC-540細胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 1215: Jacobs BB, Huseby RA. Transplantable Leydig cell tumors in Fischer rats: hormone responsibility and hormone production. J. Natl. Cancer Inst. 41: 1141-1153, 1968. PubMed: 5688092