Daoy細(xì)胞

年限： 4 years

是否是腫瘤細(xì)胞： 1

物種來源： 人

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

細(xì)胞形態(tài)： 多邊形

組織來源： cerebellum

ATCC Number： HTB-186?

器官來源： 大腦

相關(guān)**： 其他**

運(yùn)輸方式： 凍存運(yùn)輸

Designations: Daoy

Daoy細(xì)胞Depositors: HS Friedman

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: polygonal

Source: Organ: brain

Tissue: cerebellum

Disease: desmoplastic cerebellar medulloblastoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1985

Applications: Daoy細(xì)胞transfection host (Roche Transfection Reagents)

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 13,14

D16S539: 10

D5S818: 11,13

D7S820: 8,10

THO1: 9

TPOX: 8,10

vWA: 14,20

Cytogenetic Analysis: This is a hypertetraploid human cell line with a modal number between 93 and 99. The frequency of cells with higher ploidies is 2.0%. However, since the stemline chromosome number is high, the estimate for the polyploidy is tentative. Thirteen or more marker chromosomes were common to all cells. Daoy細(xì)胞Of these, many had two to four copies per cell. Among the markers were: t(1q5q), t(13q;?), 15p+, 7q+, der(9)t(3;9)(p21;q34) and eight others. In most cells, the 15p+ has three copies and der(9) has four copies.Some cells have del(1)(p11). Normal N12, N14, N15 and N19 tend to have four or more copies per cell. There are two normal X chromosomes in most cells, but there is no detectable normal Y.

Isoenzymes: AK-1, 1

ES-D, 1-2

G6PD, B

GLO-I, 1

Me-2, 1

PGM1, 2

PGM3, 1-2

Age: 4 years

Gender: male

Ethnicity: Caucasian

Comments: Daoy細(xì)胞The Daoy cell line was established in 1985 by P. F Jacobsen of the Royal Perth Hospital in Western Australia.

The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy.

Although the original tumor had characteristics of both neuronal and glial differentiation, these were not retained by the cell line.

Treatment of the cells with dibutyryl cyclic amp (cAMP) does not induce expression of those characteristics as measured by staining for S100 (S-100) protein and glial fibrillary acidic proteins (GFAP).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: Daoy細(xì)胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 34 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 23092: He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373

23156: Jacobsen PF, et al. Establishment of a human medulloblastoma cell line and its heterotransplantation into nude mice. J. Neuropathol. Exp. Neurol. 44: 472-485, 1985. PubMed: 2993532

32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024