Caov-4細胞

生長狀態(tài)： 貼壁生長

細胞形態(tài)： 上皮樣

ATCC Number： HTB-76?

相關(guān)**： 腺癌

器官來源： 卵巢

是否是腫瘤細胞： 1

物種來源： 人

運輸方式： 凍存運輸

年限： 45 years

數(shù)量： 大量

Caov-4細胞Designations: Caov-4

Depositors: J Fogh

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: ovary

Disease: adenocarcinoma

Derived from metastatic site: subserosa of the fallopian tube

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Caov-4細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

Cytogenetic Analysis: (P5) hypotriploid and hypertetraploid with abnormalities including breaks, dicentrics, minute chromatin bodies, fragmentations, secondary constrictions and large subtelocentric and submetacentric markers

Isoenzymes: AK-1, 1

ES-D, 2

G6PD, B

GLO-I, 1-2

Me-2, 2

PGM1, 1-2

PGM3, 2

Age: 45 years

Gender: female

Ethnicity: Caucasian

Propagation: Caov-4細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Temperature: 37.0°C

Atmosphere: air, 100%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).

Add fresh medium, aspirate and dispense into new flasks.

Subculture every 6 to 8 days.

Preservation: Freeze medium: Caov-4細胞complete growth medium supplemented with an additional 40% fetal bovine serum and 10% (v/v) DMSO