MeWo細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來源： 人

年限： 78 years

數(shù)量： 大量

細(xì)胞形態(tài)： 成纖維樣

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

器官來源： 皮膚

MeWo細(xì)胞ATCC Number： HTB-65?

相關(guān)**： 惡性黑色素瘤

Designations: MeWo

Depositors: Y Kodera

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens deposited as human

Morphology: fibroblast

Source: Organ: skin

Disease: MeWo細(xì)胞malignant melanoma

Derived from metastatic site: lymph node

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Antigen Expression: Blood type A; HLA A2, A26, Bw16, 18

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 12

D13S317: 8,9

D16S539: 10,12

D5S818: 12,13

D7S820: 10,12

THO1: 7,9

TPOX: 8,10

vWA: 15

MeWo細(xì)胞Isoenzymes: ACP1, B [22551]

AK-1, 1 [22551]

ES-D, 1 [22551]

G6PD, B [22551]

GLO-I, 2 [22551]

PGM1, 1-2 [22551]

PGM3, 1 [22551]

Age: 78 years

Gender: male

Ethnicity: White

Comments: The MeWo cell line was initiated by Y. Kodera and M. Bean in 1974 from lymph node tissue.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. MeWo細(xì)胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474

23256: Carey TE, et al. MeWo細(xì)胞Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619