Tera-1細(xì)胞
細(xì)胞形態(tài)： 上皮樣
年限： 47 years *****
器官來源： 其他
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 貼壁生長
數(shù)量： 大量
ATCC Number： HTB-105?
相關(guān)**： 其他**
是否是腫瘤細(xì)胞： 1
物種來源： 人
Designations: Tera-1
Depositors: J Fogh
Tera-1細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: testes
Disease: embryonal carcinoma, malignant
Derived from metastatic site: lung
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Tera-1細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Tumorigenic: No
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 10,12
D5S818: 11,13
D7S820: 9,10
THO1: 7,8
TPOX: 8
vWA: 14
Tera-1細(xì)胞Cytogenetic Analysis: at passage 20, hypotriploid (+A, +A3, +B, +C, +D, +E, +F, -A2) with abnormalities including endoreduplications, acrocentric fragments, minutes, translocations, secondary constrictions, breaks and large metacentric markers
Isoenzymes: AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 1-2
Me-2, 1-2
PGM1, 1
PGM3, 1-2
Age: 47 years *****
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: Tera-1細(xì)胞A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Subculture every 6 to 8 days.
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
32279: Schar BK, et al. Simultaneous detection of all four alkaline phosphatase isoenzymes in human germ cell tumors using reverse transcription-PCR. Cancer Res. 57: 3841-3846, 1997. PubMed: 9288797
