HCC1187細胞

運輸方式： 凍存運輸

生長狀態(tài)： 混合型生長

數(shù)量： 大量

組織來源： duct

細胞形態(tài)： 上皮樣

是否是腫瘤細胞： 1

物種來源： 人

年限： TNM stage IIA, grade 3

ATCC Number： CRL-2322?

相關**： 導管癌

器官來源： **

HCC1187細胞Designations: HCC1187

Depositors: AF Gazdar, AK Virmani

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Tissue: duct

Tumor Stage: TNM stage IIA, grade 3

Disease: primary ductal carcinoma

Cellular Products: Epithelial glycoprotein 2 (EGP2)

HCC1187細胞cytokeratin 19

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

Applications: HCC1187 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.

The cells are negative for expression of Her2-neu; they overexpress p53.

The cells are negative for expression of progesterone receptor (PR).

The cells are poorly differentiated.

Receptors: progesterone receptor, negative

Oncogene: her2/neu -, p53 +

DNA Profile (STR): HCC1187細胞Amelogenin: X

CSF1PO: 13

D13S317: 11

D16S539: 10

D5S818: 12

D7S820: 8,11

THO1: 6

TPOX: 8

vWA: 19

Cytogenetic Analysis: multiploid; cell population has several ploidy indices

Age: 41 years *****

Gender: female

Ethnicity: Caucasian, White

Comments: This cell line was initiated on 9/13/94 from a patient who had received prior chemotherapy. The cell line took 4.5 months to establish.

The tumor was classified as TNM stage IIA, grade 3, invasive ductal carcinoma. Information regarding lymph node metastasis is not available.

The cells are poorly differentiated.

The cells are negative for expression of Her2-neu; they overexpress p53.

HCC1187 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.

HCC1187細胞The cells are negative for expression of progesterone receptor (PR).

An EBV transformed lymphoblastoid cell line (HCC1187 BL) from the same patient is available as ATCC CRL-2323.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. Attached cells may be subcultured using 0.25% trypsin-0.53 mM EDTA.

Cultures grow as floating aggregates of round cell clusters and as attached epithelial-like cells.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

normal (or near-normal) cell line established from the same patient:ATCC CRL-2323

purified DNA:HCC1187細胞ATCC CRL-2322D

purified RNA:ATCC CRL-2322R

References: 38266: Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771