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KHOS-240S細(xì)胞

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產(chǎn)品名稱: KHOS-240S細(xì)胞
產(chǎn)品型號(hào): KHOS-240S
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

KHOS-240S細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。KHOS-240S細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


KHOS-240S細(xì)胞  的詳細(xì)介紹

KHOS-240S細(xì)胞

ATCC Number: CRL-1545?

相關(guān)**: 骨肉瘤

細(xì)胞形態(tài): 成纖維樣

數(shù)量: 大量

是否是腫瘤細(xì)胞: 1

物種來源: 人

生長狀態(tài): 貼壁生長

年限: 13 years

運(yùn)輸方式: 凍存運(yùn)輸

器官來源: 骨

KHOS-240S細(xì)胞Designations: KHOS-240S

Depositors: JS Rhim

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast


Source: Organ: bone

Disease: osteosarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: No

DNA Profile (STR): KHOS-240S細(xì)胞Amelogenin: X

CSF1PO: 12

D13S317: 12

D16S539: 10,13

D5S818: 13

D7S820: 11,12

THO1: 6

TPOX: 11

vWA: 18

Age: 13 years

Gender: female

Ethnicity: Caucasian

Comments: The growth properties of KHOS-240S are similar to HOS (ATCC CRL 1543).

This line is a revertant of ATCC CRL-1544 that no longer has a rescuable Kirsten murine sarcoma virus genome.

Propagation: KHOS-240S細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 3 to 5 minutes or until cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 days.

References: 22503: Yang YH, et al. Reversion of Kirsten sarcoma virus transformed human cells: elimination of the sarcoma virus nucleotide sequences. J. Gen. Virol. 43: 447-451, 1979. PubMed: 225428

26080: Cho HY, et al. Revertants of human cells transformed by murine sarcoma virus. Science 194: 951-953, 1976. PubMed: 62397

26082: Cho HY, Rhim JS. KHOS-240S細(xì)胞Cycloheximide-dependent reversion of human cells transformed by MSV and chemical carcinogen. Science 205: 691-693, 1979. PubMed: 223242

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