NCI-H735細(xì)胞

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

器官來(lái)源： 肺

ATCC Number： CRL-5978?

相關(guān)**： 小細(xì)胞肺癌

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

年限： stage E

運(yùn)輸方式： 凍存運(yùn)輸

Designations: NCI-H735 [H735]

NCI-H735細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Tumor Stage: stage E

Disease: carcinoma; small cell lung cancer

Derived from metastatic site: liver

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: NCI-H735細(xì)胞The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12,14

D13S317: 11,14

D16S539: 14

D5S818: 9,11

D7S820: 8,11

THO1: 8,9.3

TPOX: 11

vWA: 18,19

Age: 58 years

Gender: female

NCI-H735細(xì)胞Ethnicity: Caucasian

Comments: The line was established in August 1984.

The patient received prior chemotherapy.

The patient was a smoker.

60 pack years.

Propagation: ATCC complete growth medium: HITES medium (serum free) HITES medium is formulated at the ATCC as follows: Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006) Insulin 0.005 mg/ml Transferrin 0.01 mg/ml Sodium selenite 30 nM Hydrocortisone 10 nM beta-estradiol 10 nM HEPES 10 mM L-glutamine 2 mM (in addition to that in the base medium)

Temperature: 37.0°C

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

References: 23570: . NCI-H735細(xì)胞NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.