97人妻人人做人碰人人爽,亚洲AV无码一区二区乱孑伦AS,免费人成视频在线观看 ,99久久精品日本一区二区免费

產(chǎn)品資料

NCI-H2029細(xì)胞

如果您對(duì)該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱: NCI-H2029細(xì)胞
產(chǎn)品型號(hào): NCI-H2029
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔

簡(jiǎn)單介紹

NCI-H2029細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o(wú)菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無(wú)菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。NCI-H2029細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


NCI-H2029細(xì)胞  的詳細(xì)介紹

NCI-H2029細(xì)胞

數(shù)量: 大量

器官來源: 肺

年限: stage E

是否是腫瘤細(xì)胞: 1

物種來源: 人

ATCC Number: CRL-5913?

相關(guān)**: 小細(xì)胞肺癌

生長(zhǎng)狀態(tài): 貼壁生長(zhǎng)

運(yùn)輸方式: 凍存運(yùn)輸

Designations: NCI-H2029 [H2029]

Depositors: AF Gazdar, JD Minna

NCI-H2029細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Tumor Stage: stage E

Disease: carcinoma; small cell lung cancer

Derived from metastatic site: lymph node

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: NCI-H2029細(xì)胞The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10, 11

D13S317: None detected

D16S539: 12

D5S818: 10, 11

D7S820: 9, 11

THO1: 6, 9.3

TPOX: 8

vWA: 15, 20

Age: 69 years

Gender: female

Ethnicity: Caucasian

Comments: The line was established in September 1988.

The patient received prior chemotherapy and radiation therapy.

NCI-H2029細(xì)胞The patient was a smoker.

52 pack years.

Propagation: ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum

The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium

0.005 mg/ml Insulin

0.01 mg/ml Transferrin

30nM Sodium selenite (final conc.)

10 nM Hydrocortisone (final conc.)

10 nM beta-estradiol (final conc.)

extra 2mM L-glutamine (for final conc. of 4.5 mM)

5% fetal bovine serum (final conc.)


Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.

Preservation: NCI-H2029細(xì)胞culture medium, 90%; additional fetal bovine serum, 5%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

Recommended serum: ATCC 30-2020

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

滬公網(wǎng)安備 31011702004356號(hào)

传家在线观看免费观看完整版| 国产精品久久久久久久久久免费| 公和我边做饭边做好爽| 国内精品久久久久久久小说| 我和黑帮老大的365天| 动漫内衣办公室| 一本久道综合色婷婷五月| 啊灬啊灬啊灬啊灬快灬高潮了| 色欲av| 人妻 校园 激情 另类| 国产精品人成视频免费播放| 亚洲色少妇熟女11p| 亚洲AV成人无码网天堂| 精品久久香蕉国产线看观看亚洲| 女同学浮乱系列合集| 局长含了一整晚我的奶头| 久久久久久久久精品无码中文字幕| 国产精品高清一区二区三区人妖| 《善良的小峓子在钱完整版韩剧》| 国产99久久久久久免费看| 精品少妇一区二区三区视频 | 把女人弄爽特黄a大片视频 | 供人泄欲玩弄的妓女h| 第一次玩老妇真实经历| 亚洲日韩国产一区二区三区| 亚洲成aV人片在线观看WWW| 日本a片| 竹菊精品久久久久久久99蜜桃| 国产精品久久久久久久9999| 特级做A爰片毛片免费看无码| 亚洲AV日韩AV永久无码久久| 白天躁晚上躁天天躁2022| 扒开她粉嫩的小缝尿进去h漫画| 真实的国产乱XXXX在线| 久久精品国产精品亚洲| 精品无码国产污污污免费网站| 人妻丝袜无码国产一区| 小白兔进化史| 一本久道久久综合狠狠爱| 我和岳m愉情xxxx国产| 女沟厕偷窥piss小便|